Fate of uptaken host proteins in Taenia solium and Taenia crassiceps cysticerci

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Production of monoclonal antibodies anti-Taenia crassiceps cysticerci with cross-reactivity with Taenia solium antigens.

We describe the production of the potential monoclonal antibodies (MoAbs) using BALB/c mice immunized with vesicular fluid (VF)-Tcra (T. crassiceps) antigen. Immune sera presented anti-VF-Tcra (<20kD) IgG and IgM antibodies with cross-reactivity with T. solium (Tso) antigen (8-12, 14, and 18 kD). After cell fusion, we selected 33 anti-Tcra and anti-Tso reactive IgM-clones and 53 anti-Tcra speci...

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ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci.

In the present study ELISA was standardized for the diagnosis of swine cysticercosis based on necropsy parameters and confirmed positive and negative control sera. Serum samples from pigs with other infections were also assayed to determine possible cross-reactions. Four antigens were assayed: from Taenia crassiceps vesicular fluid (VF-Tcra) and crude larvae extract (T-Tcra), and from Taenia so...

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Experimental encephalitis caused by Taenia crassiceps cysticerci in mice.

OBJECTIVES To present the experimental model of neurocysticercosis (NCC) caused by Taenia crassiceps cysticerci, to describe the inflammatory process, susceptibility, or resistance of BALB/c and C57BL/6 mice to this infection, and to describe the host-parasite relationship. METHODS The animals were intracranially inoculated with initial stage T. crassiceps cysticerci. They were euthanized at ...

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Mitochondrial Thioredoxin-Glutathione Reductase from Larval Taenia crassiceps (Cysticerci)

Mitochondrial thioredoxin-glutathione reductase was purified from larval Taenia crassiceps (cysticerci). The preparation showed NADPH-dependent reductase activity with either thioredoxin or GSSG, and was able to perform thiol/disulfide exchange reactions. At 25 degrees C specific activities were 437 +/- 27 mU mg(-1) and 840 +/- 49 mU mg(-1) with thioredoxin and GSSG, respectively. Apparent K(m)...

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Principles of Variation of Energy Metabolism in Taenia Solium cysticerci

Metabolites and enzyme activities involved in energy metabolism in Taenia solium cysticerci were determined systematically in vivo in order to illustrate the principles of variation of energy metabolism. Metabolites and enzymes determined included GLC, LAC, PEPCK, PK, LDH, SDH, ICD, XOD, ME, GDH, FR, ATPase, ACP, AKP, G6Pase and FE. The results showed that transport of substances, anaerobic gly...

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ژورنال

عنوان ژورنال: Bioscience Reports

سال: 2018

ISSN: 0144-8463,1573-4935

DOI: 10.1042/bsr20180636